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Alpha-fetoprotein (AFP) immunoradiometric assay kit

Alpha-fetoprotein (AFP) immunoradiometric assay kit RIA

The OCFA07-AFP kit is a rapid immunoradiometric assay for alpha-fetoprotein (AFP) in serum or plasma for diagnosis and monitor many pathologies.

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The OCFA07-AFP kit is a rapid immunoradiometric assay for alpha-fetoprotein (AFP) in serum or plasma for diagnosis and monitor many pathologies.

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Overview

Diagnosis and monitor AFP-producing tumors in the testes

Diagnose and monitor in liver disorders: hepatitis, cirrhosis of the liver and liver cell carcinoma

Screen during pregnancy to detect open neural tube defect or an anencephaly

Benefits

FAST 30 MIN ASSAY

Assay principle: sandwich

Two monoclonal antibodies are used for the sandwich. The first one is adsorbed onto the tube walls, the second one is radiolabeled with iodine-125 and used as a tracer.

Assay procedure: 15 min at RT / shaking + 15 min at RT / shaking.

Features

Working range: 0.3 – 800 IU/mL (1st IS for AFP 72/225)

Sample: serum or plasma – 50 µL

Analytical sensitivity: 0.3 IU/mL

Normal values: tumor diagnosis

n = 298 healthy subjects, 95th percentile <= 5 IU/mL

Normal values: screening during pregnancy

Table 1: Standard values for maternal serum AFP concentrations between the 14th and 21st week of pregnancy (The definition of gestational age varies for different countries).

Current weeks of gestation	14^th	15^th	16^th	17^th	18^th	19^th	20^th
Completed	15^th	16^th	17^th	18^th	19^th	20^th	21^st
n	116	1 587	4 112	3 092	2 038	1 065	254
Median (IU/mL)	21	27.5	31	37	42	48	56.5
2.5-fold Median (IU/mL)	52.5	69	77.5	92.5	105	120	141

Current week of pregnancy	15^th	16^th	17^th	18^th	19^th	20^th	21^st
n	61	119	154	88	49	43	38
Median (IU/mL)	10 620	9 440	8 220	7 750	5 310	5 530	4 780
3-fold Median (IU/mL)	31 860	28 320	24 660	23 250	15 930	16 590	14 340