Total CREB cellular kit
Simple and robust detection kit for Phospho CREB & Total CREB
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The phospho-CREB (S133) kit is optimal for the quantitative detection of the cyclic AMP-responsive element-binding protein when phosphorylated at S133, thus enabling the phosphorylation status of CREB to be checked with respect to its steady-state level in cells. CREB functions as a transcription factor, signaling downstream from MAPK/ERK and activated by several pathways, such as MAPK/ERK, PI3K/AKT and stress signaling. CREB plays a critical role in promoting neuronal plasticity, cognitive abilities, cell proliferation, survival, and glucose homeostasis.
Mouse pancreatic ß-cells Min6 are seeded in culture-treated 96-well plates at 70Kc/well and cultured for 6 days in complete culture medium at 37°C, 5% CO2. After a cell washing step with KRB (Krebs Ringer Buffer) and a quiescence step for 2h in KRB, cells were stimulated with 50 µL KRB and increasing concentrations of Glucose for 5 min at 37°C. After a 30 minutes lysis incubation time, total and phospho-CREB were measured using the two-plate assay protocol.
Murine NIH 3T3 cells (50,000 cells/well) were stimulated for 30 minutes at 37°C with various concentrations of forskolin. After a 30 minutes lysis incubation time, phosphorylated CREB was measured using the two-plate assay protocol.
A431 cells (100,000 cells/well) were incubated for 30 min at 37°C with various concentrations of antagonist. Agonist (EGF) was then added and incubated for 10 minutes. After 30 minutes lysis incubation time, phosphorylated CREB was measured using the two-plate assay protocol.
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Changes in CREB and ERK phosphorylation levels after beta-amyloid treatment - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
Multidimentional GPCR investigations - Posters
TCR signaling investigation with HTRF phospho assays
Study a pathway of interest in PBMC and T cells - Application Notes
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
HTRF and Tag-lite technologies in multidimensional cell-based assays for GPCR investigations
16 GPCR challenges, 1 solution - Posters
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Videos
Product Insert CREB P-S133 Kit / 64CREPET
64CREPET - Product Insert
Product Insert CREB P-S133 Kit / 64CREPEG-64CREPEH
64CREPEG-64CREPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
An innate and adaptive immunity recap
Insight into the diversity of cells & signaling pathways - Guides
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet (DEU) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (ELL) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (ITA) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (SPA) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) CREB P-S133 Kit / 64CREPEG
64CREPEG - Safety Data Sheet
Safety Data Sheet (DEU) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (ELL) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (ITA) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (SPA) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) CREB P-S133 Kit / 64CREPEH
64CREPEH - Safety Data Sheet
Safety Data Sheet (ELL) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (FRA-FR) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (ITA) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (SPA) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (ENG-GB) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (ENG-US) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (DEU) CREB P-S133 Kit / 64CREPET
64CREPET - Safety Data Sheet
Safety Data Sheet (DEU) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
Safety Data Sheet (ELL) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
Safety Data Sheet (ITA) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
Safety Data Sheet (SPA) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
Safety Data Sheet (ENG-US) CREB P-S133 Kit / 64CREPEY
64CREPEY - Safety Data Sheet
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