SARS-Cov2 nucleocapsid detection kit HTRF®

This HTRF kit is designed to detect SARS-CoV-2 nucleocapsid proteins in cell lysates or cell supernatant, as a marker of viral infection.

See more
  • No-wash No-wash
  • High sensitivity High sensitivity

This HTRF kit is designed to detect SARS-CoV-2 nucleocapsid proteins in cell lysates or cell supernatant, as a marker of viral infection.

In stock


The SARS-CoV-2 N protein is the basal monomeric constituent of the coronavirus oligomeric nucleocapsid. This protein is major immunogen predominantly expressed at early stages of viral infection and present from day one in infected patients’ serum. As such, SARS-CoV-2 N protein is a reliable marker for viral infection and a possible immunogen candidate for the development of a vaccine.

SARS-CoV-2 particles are detected in cell lysates using a sandwich immunoassay involving an anti-nucleocapsid monoclonal antibody, labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of nucleocapsid proteins.
Principle of the SARS-CoV-2 nucleocapsid assay
The assay protocol, using a 384-well small volume plate or a low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure (4 µL), or separately (2 µL).
Protocol of the SARS-CoV-2 nucleocapsid assay

Detection of recombinant SARS-CoV-2 nucleocapsid

16 µL of recombinant protein in lysis buffer #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after 2h incubation at RT.
SARS-CoV-2 S1 protein standard curve

Standard curve Capsid kit

Sample size

16 µL
Final assay volume20 µL
Time to results2h at RT
Detection limit165 pg/mL
Dynamic range307 to 75,000 pg/mL
Sample compatibilityCell-based or cell supernatant
Nucleocpasid standard curve

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Catalogue produits IVD 2020

Immuno-analyse RIA et ELISA - Catalog

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

An innate and adaptive immunity recap

Insight into the diversity of cells & signaling pathways - Guides

Innovative solutions in Virology research

Kits and reagents for virology research - Flyers

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

Let's find your reader