Two screen islet cell autoantibody (GADAb & IA-2Ab) ELISA kit ELISA
Use as serological markers of type 1 diabetes mellitus (insulin-dependent diabetes mellitus - IDDM)
- ACCURATE & RELIABLE ENZYMATIC ASSAY
GAD65 and IA-2 molecules are coated onto the ELISA plate wells. GAD65-biotin and IA-2-biotin complexes constitute the liquid phase. GAD-Ab and IA-2-Ab from the sample bind to the GAD65 and to IA-2 immobilized onto the well and interact with GAD65-biotin and IA-2 biotin via a GAD-Ab and IA-2-Ab bridges. Addition of streptavidin peroxidase detects the bound GAD65-biotin and IA-2 biotin.
The unbound streptavidin peroxidase in excess is washed off before the addition of the peroxidase substrate TMB. Assay procedure: 16-20h at 2-8°C + 1h at RT / shaking + 20 min at RT / shaking + 20 min at RT in the dark.
Working range: 4 - 450 U/mL (NIBSC 97/550)
Sample: serum - 50 µL Analytical sensitivity: 0.17 U/mL